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In this tech tip, we describe how careful adherence to just a few basic procedures can help to ensure reliable absorbance measurements.
Absorbance is used as a qualitative tool to identify substances or a quantitative tool to measure the concentration of a molecule in solution. With modular spectroscopy, users choose the wavelength range and resolution best suited for their measurements, and can move between setups for the lab or field. Preconfigured absorbance measurement packages are also available.
Once you’ve selected your absorbance setup, follow these four procedures to ensure best results:
No matter what specific biological test one may be dealing with, UV absorbance is commonly used to determine the concentration of a constituent.