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Absorbance Measurement
From UV to NIR and longer wavelengths, absorbance measurements provide valuable information about the chemical composition of materials in all states of matter.
Light incident on a sample can be transmitted, absorbed or scattered. Transmission is the light that passes through the sample; light that encounters a molecule can be absorbed or scattered. Absorbance can be used as a qualitative tool to identify substances, or as a quantitative tool to measure the concentration of a molecule in solution.
Advantages of Absorbance
- Non-destructive measurements - Unless your sample is photosensitive, the measurement can be repeated without altering the sample.
- Quantitative results - Use absorbance to determine the concentration of solutions.
- Accuracy and repeatability - With proper tools and methods, absorbance can be quantified with great measurement-to-measurement precision.
Absorbance of Solutions
Configuring a setup for absorbance begins with a general-purpose spectrometer like the Ocean HDX. Complete the system with a light source, fibers and cuvette- or probe-based sampling optics.
Case Study
App Notes
Product Sheet
This 1 cm pathlength cuvette holder is ideal for accurate, repeatable absorbance and fluorescence measurements. Square One provides a snug, reliable fit for cuvettes and filters. Download Product Sheet to learn more!
