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Absorbance Measurement

Absorbance Measurement Technnique

From UV to NIR and longer wavelengths, absorbance measurements provide valuable information about the chemical composition of materials in all states of matter.

Light incident on a sample can be transmitted, absorbed or scattered. Transmission is the light that passes through the sample; light that encounters a molecule can be absorbed or scattered. Absorbance can be used as a qualitative tool to identify substances, or as a quantitative tool to measure the concentration of a molecule in solution.


Advantages of Absorbance

  • Non-destructive measurements - Unless your sample is photosensitive, the measurement can be repeated without altering the sample.
  • Quantitative results - Use absorbance to determine the concentration of solutions.
  • Accuracy and repeatability - With proper tools and methods, absorbance can be quantified with great measurement-to-measurement precision.
Scientific Background Protein

Absorbance of Solutions

Configuring a setup for absorbance begins with a general-purpose spectrometer like the Ocean HDX. Complete the system with a light source, fibers and cuvette- or probe-based sampling optics.Absorbance -Illustration.png

Case Study

App Notes

Product Sheet

This 1 cm pathlength cuvette holder is ideal for accurate, repeatable absorbance and fluorescence measurements. Square One provides a snug, reliable fit for cuvettes and filters. Download Product Sheet to learn more!

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