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Fluorescence is the absorption and emission of light of two different frequencies. Typically, a lower wavelength of incident light is absorbed from one direction, and a higher wavelength of light is emitted in all directions.
Fluorescent molecules absorb light at a certain wavelength and emit at another. With a known incident light wavelength, samples can be identified by their fluorescent emission spectra. Because fluorescence occurs on a molecular scale, it is the only spectroscopic technique capable of identifying single molecules.
Quantitative assessment of advanced glycation end products in the eye lens can be realized by using fluorescence spectroscopy.