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Fluorescence Measurement
Fluorescence is the absorption and emission of light of two different frequencies. Typically, a lower wavelength of incident light is absorbed from one direction, and a higher wavelength of light is emitted in all directions.
Fluorescent molecules absorb light at a certain wavelength and emit at another. With a known incident light wavelength, samples can be identified by their fluorescent emission spectra. Because fluorescence occurs on a molecular scale, it is the only spectroscopic technique capable of identifying single molecules.
Advantages of Fluorescence
- High sensitivity - Very low sample concentration levels can be detected
- Quantitative results - In most cases, fluorescence signal is proportional to concentration and responds to concentration changes within picoseconds
- Non-destructive measurements - Unlike some techniques, fluorescence is non-destructive to both liquid and solid samples
Case Study & App Notes
Quantitative assessment of advanced glycation end products in the eye lens can be realized by using fluorescence spectroscopy.
